Table 2.

Overview of blood sampling for FVIII:C measurements

	Within 4 weeks before surgery*	D0 = day of first desmopressin infusion				D1				D2		D3	4–8 weeks after FVIII treatment
Time–bolus infusions†	Baseline	Pre	Post	Peak	Post-treat	Pre	Post	Peak	Pre	Post	Peak	Trough
Time–continuous infusion‡	Baseline	Pre	Post	Peak	Post-treat	Pre	Post	Preadjust	Pre	Post	Preadjust	Steady state
Primary endpoint						X			X			X
Sodium	X	X				X			X			X
Neutralising antibodies	X												X

Grey indicates obligatory measurements.

FVIII measurements are shown here until day 3 after start of combination treatment. Monitoring will continue if treatment is still necessary.

*Only in case of elective surgery, otherwise before first desmopressin administration.

†Pre, before desmopressin; post, after desmopressin; peak, after FVIII concentrate; post-treat=after surgery; in case of bleeding: 2–6 hours after desmopressin, trough, before next dose of FVIII concentrate.

‡Pre, before desmopressin; post, after desmopressin; peak, after loading dose FVIII concentrate; post-treat, after surgery; in case of bleeding: 2–6 hours after desmopressin; preadjust, before dosage; steady state, FVIII measurement at random time point.