Table 1.

Reactions used for assessing O₂^•− kinetics in the phagosome

Reaction	Observation*	Source
Net O2 consumption	R1 = 0.012	†
O2 → O2•−	R2 = R1 + (R3 + R4 + R5)/2	‡
2O2•− + 2H+ → O2 + H2O2	k3 < 0.3–100	§
O2•− + HO2• + H+ → O2 + H2O2	k4 = 8.5–10 × 107	§
2HO2• → O2 + H2O2	k5 = 7.6–8.6 × 105	§
O2•− + H+ ⇌ HO2•	pKa = 4.69–4.88	§

^*Rates (R) are in M/s. First- and second-order rate constants (k_i) are in s⁻¹ and M⁻¹⋅s⁻¹, respectively.

^†Calculated as detailed in Materials and Methods.

^‡The rate of NOX-2 O₂^•− production is the sum of the net O₂ consumption and half of the spontaneous dismutation rate.

^§Behar et al. (41), Bielski (42), and Bielski and Allen (43).