Table 1.
Reaction | Observation* | Source |
Net O2 consumption | R1 = 0.012 | † |
O2 → O2•− | R2 = R1 + (R3 + R4 + R5)/2 | ‡ |
2O2•− + 2H+ → O2 + H2O2 | k3 < 0.3–100 | § |
O2•− + HO2• + H+ → O2 + H2O2 | k4 = 8.5–10 × 107 | § |
2HO2• → O2 + H2O2 | k5 = 7.6–8.6 × 105 | § |
O2•− + H+ ⇌ HO2• | pKa = 4.69–4.88 | § |
Rates (R) are in M/s. First- and second-order rate constants (ki) are in s−1 and M−1⋅s−1, respectively.
Calculated as detailed in Materials and Methods.
The rate of NOX-2 O2•− production is the sum of the net O2 consumption and half of the spontaneous dismutation rate.