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. Author manuscript; available in PMC: 2019 May 4.
Published in final edited form as: Cell Rep. 2018 May 15;23(7):2083–2094.e6. doi: 10.1016/j.celrep.2018.04.060

Figure 1. BOK-Mediated Membrane Permeabilization Correlates with Its Metastability.

Figure 1.

(A) Human BOK constructs used in this study. The BCL-2 homology regions (BH1–BH4), TM, residue numbers, and tags are indicated for BOK constructs.

(B) LUVP measured as fluorescent dye release by FL-BOK-DTM-CH was normalized relative to permeabilization by 2.5% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate (CHAPS). Normalized data were analyzed by Simpson’s rule AUC at 32 min. Data represent the average and SD for one of three experiments performed in triplicate.

(C) Combined average and SE AUC at 32 min for the BOK constructs. Related to Figure S1C.

(D) Combined average and SE of BOK concentration required to achieve half-maximal AUC at 32 min for the BOK constructs (related to Figure S1D).

(E) MOMP assays of purified bak−/− mitochondria were performed for 1 hr at 37°C for monitoring cyt c release and protein loading.

(F) Thermal denaturation measured by TSA at 0.25 mg/mL protein was performed using the manufacturer’s buffer conditions. Data represent average melting temperature (Tm) and SD of replicate performed in quadruplicate.

See also Figure S1.