KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Experimental Models: Strains/Cell Lines | ||
| Escherichia coli strain: XL110-Gold | Agilent Technologies | Cat# C404010 |
| Escherichia coli strain: BL21 Star (DE3) | Thermo Fisher Scientific | Cat# C601003 |
| Escherichia coli strain: T7 Express | New England Biolabs | Cat# C2566I |
| Escherichia coli strain: T7 Express lysY/Iq | New England Biolabs | Cat# C3013I |
| Phoenix Ampho Packaging Cells | ATCC | Cat# CRL-3213 |
| Immortalized SV40 bak−/−bax−/− Mouse Embryonic Fibroblasts | (Llambi et al., 2016) | PMID 26949185 |
| Bak−/− mice | Jackson Laboratories | B6.129-Bak1tm1Thsn/J |
| Peptides | ||
| hBID_BH3: EDIIRNIARHLAQVGDSBDRSI (B = Nle) | This paper | N/A |
| hBID_BH3a: EDIIRNAARHLAQVGDSMDRSI | This paper | N/A |
| hBID_BH3b: EDIIRNIARHAAQVGDSMDRSI | This paper | N/A |
| hBID_BH3c: EDIIRNAARHAAQVGDSMDRSI | This paper | N/A |
| hBID_BH3d: EDIIRNIARHLWQVGDSMDRSI | This paper | N/A |
| hBID_BH3e: EDIIRNAARHLWQVGDSMDRSI | This paper | N/A |
| hBID SAHB: EDIIRNIARHLAXVGDXBDRSI (X = pentenyl alanine) | This paper | N/A |
| hBOK BH3: RLAEVCAVLLRLGDELEBIR | This paper | N/A |
| hBOK SAHB: RLAEVSAVLLXLGDXLEBIR | This paper | N/A |
| Recombinant DNA | ||
| pRetroX-TRE3G | Clontech | Cat# 631188 |
| pcDNA3.1 | Invitrogen | Cat# V79020 |
| pEGFP N1 | Clontech | Cat# 6085–1 |
| pNIC28-Bsa4 | (Savitsky et al., 2010) | Addgene #26103 |
| Human BOK and mutants in pNIC28-Bsa4 | This paper | N/A |
| Mouse BOK and mutants in pRetroX-TRE3G | (Llambi et al., 2016); this paper | PMID 26949185; N/A |
| FLAG-human tBID and mutants in pcDNA3.1 | (Llambi et al., 2011); this paper | PMID 22036586; N/A |
| Human tBID-mCherry in pEGFP N1 | (Llambi et al., 2011) | PMID 22036586 |
| Chemicals | ||
| Fetal Bovine Serum - Premium Select | Atlanta Biologicals | Cat# S11550 |
| DMEM, high glucose, no phosphates | Thermo Fisher Scientific | Cat# 11971025 |
| GIBCO L-glutamine | Thermo Fisher Scientific | Cat# 25030081 CAS# 56-85-9 |
| GIBCO Penicillin-Streptomycin | Thermo Fisher Scientific | Cat# 15140122 CAS# 69-57-8, 57-92-1 |
| GIBCO Sodium Pyruvate | Thermo Fisher Scientific | Cat# 11360070 CAS# 113-24-6 |
| GIBCO MEM Non-Essential Amino Acids Solution | Thermo Fisher Scientific | 11140050 |
| GIBCO 2-Mercaptoethanol | Thermo Fisher Scientific | Cat# 21985023 CAS# 60-24-2 |
| 0.25% Trypsin, 0.1% EDTA in HBSS w/o Calcium, Magnesium and Sodium Bicarbonate | Corning | 25053CI |
| Lipofectamine 3000 | Thermo Fisher Scientific | Cat# L3000015 |
| Zeocine | Thermo Fisher Scientific | Cat# R25001 |
| Blastocidine S HCl powder | Thermo Fisher Scientific | Cat# R21001 |
| Doxycycline | Clontech | Cat# 631311 CAS# 24390-14-5 |
| Z-Leu-Leu-Leu-al (MG132) | Sigma Aldrich | Cat# C2211 |
| CAS# 133407-82-6 | ||
| SYTOX Green | Thermo Fisher Scientific | Cat# S7020 CAS# 163795-75-3 |
| Q-VD-Oph hydrate | APExBIO | Cat# A1901 |
| Propidium Iodide | Invitrogen | Cat# P3566 CAS# 25535-16-4 |
| L-α-phosphatidylcholine (Egg, Chicken) | Avanti Polar Lipids | Cat# 840051C CAS# 97281-44-2 |
| L-α-phosphatidylinositol (Liver, Bovine) (sodium salt) | Avanti Polar Lipids | Cat# 840042C CAS# 383907-33-3 |
| L-α-phosphatidylserine (Brain, Porcine) (sodium salt) | Avanti Polar Lipids | Cat# 840032C CAS# 383907-32-2 |
| Cardiolipin (Heart, Bovine) (sodium salt) | Avanti Polar Lipids | Cat# 840012C CAS# 383907-10-6 |
| 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl)iminodiacetic acid)succinyl] (nickel salt) | Avanti Polar Lipids | Cat# 790404C CAS# 231615-77-3 |
| 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine | Avanti Polar Lipids | Cat# 850725C CAS# 4004-05-1 |
| ANTS (8-aminonpaphthalene-1,3,6-trisulfonic acid, disodium salt) | Molecular Probes | Cat# 1278701 CAS# 5398-34-5 |
| DPX (p-xylene-bis-pyridiunium bromide) | Molecular Probes | Cat# X1525 CAS# 14208-10-7 |
| 15N Ammonium Chloride | Cambridge Isotope Laboratories | Cat# NLM-467–10 CAS# 12125-02-9 |
| 13C Glucose | Cambridge Isotope Laboratories | Cat# CLM-1396–1 CAS# 50-99-7 |
| Deuterated DTT | Cambridge Isotope Laboratories | Cat# DLM-2622–1 |
| Deuterium Oxide | Sigma Aldrich | Cat# 617385–1 CAS# 7789-20-0 |
| Deuterated DMSO | Sigma Aldrich | Cat# 151874 |
| Ponceau S | G-Biosciences | Cat# 786–576 |
| cOmplete, Mini Protease Inhibitor Cocktail | Roche | Cat# 11836153001 |
| Antibodies | ||
| Anti-cyt c monoclonal antibody | BD Biosciences | Cat# 556433; RRID:AB_396417 |
| Anti-human BID monoclonal antibody | Santa Cruz | Cat# SC-56025; RRID:AB_781628 |
| Anti-human BOK monoclonal antibody | Abcam | Cat# ab186745 |
| Anti-GFP monoclonal antibody | Santa Cruz | Cat# SC-9996; RRID:AB_627695 |
| Anti-actin monoclonal antibody | Millipore Sigma | Cat# MAB1501; RRID:AB_2223041 |
| Critical Commercial Assays | ||
| QuikChange II XL Site-Directed Mutagenesis Kit | Agilent Technologies | Cat# 200522 |
| QIAprep Spin Miniprep Kit | QIAGEN | Cat# 27106 |
| Plasmid Plus Midi Kit | QIAGEN | Cat# 12945 |
| Protein Thermal Shift Starter Kit | Thermo Fisher Scientific | Cat# 4462263 |
| Supersignal West Dura | Thermo Fisher Scientific | Cat# 34075 |
| Deposited Data | ||
| Human BOK structure | This paper | PDB: 6CKV.pdb; BioMagResBank: 304023 |
| Software and Algorithms | ||
| TopSpin v3.2 | Bruker BioSpin | http://www.bruker.com |
| CARA v1.9.1.7 | (Keller, 2004) | http://cara.nmr.ch/doku.php |
| TALOS+ | (Cornilescu et al., 1999) | https://spin.niddk.nih.gov/bax/software/TALOS/ |
| UNIO’10 v2.0.1 | (Guerry and Herrmann, 2012) | http://perso.ens-lyon.fr/torsten.herrmann/Herrmann/Software.html |
| CYANA v2.1 | (Guntert, 2004) | http://www.cyana.org |
| MOLMOL v2.1–2.6 | (Koradi et al., 1996) | https://sourceforge.net/p/molmol/wiki/Home/ |
| PROCHECK v3.4.3 | (Laskowski et al., 1996) | https://www.ebi.ac.uk/thornton-srv/software/PROCHECK/ |
| ProtSkin | (Ritter et al., 2004) | http://www.mcgnmr.mcgill.ca/ProtSkin/ |
| MacPyMOL v1.7.6.3 | Schrodinger, LLC | https://pymol.org/2/ |
| ImageJ | NIH, USA | https://www.imagej.nih.gov/ij |
| IncuCyte v2016A | Essen BioScience | https://www.essenbioscience.com |
| IncuCyte 2011A Rev2 v20111.3.4288 | Essen BioScience | https://www.essenbioscience.com |
| FlowJo Xv10.0.7r2 | FlowJo, LLC | https://www.flowjo.com |
| Microsoft Excel | Microsoft | https://products.office.com/excel?legRedir=true&CorrelationId=129ae579-ccf4–43ae-89ae-a241f392c557 |
| Prism v7.0a | GraphPad Software | https://www.graphpad.com |
| Protein Thermal Shift Software v1.3 | Thermo Fisher Scientific | Cat# 4466037 |
| ABI 7900HT Sequence Detection Systems v 2.4.1 | Thermo Fisher Scientific | Cat# 4350490 |
| ClarioSTAR Microplate Reader Software V5.40 Edition 2; Reader Control v5.40; Firmware v1.20; MARS Data Analysis v3.30 | BMG Lab Tech | https://www.bmglabtech.com/en/products/software/ |
| Other | ||
| Nickel agarose beads (high density) | Gold Biotechnology | Cat# H-320–500 |
| Amicon Ultra 15-mL 3K MWCO centrifugal filter | Millipore Sigma | Cat# UFC900324 |
| Amicon Ultra 15-mL 10K MWCO centrifugal filter | Millipore Sigma | Cat# UFC800324 |
| MonoS 5/50 GL column | GE Healthcare | Cat# 17-5168-01 |
| Superdex200 Increase 10/300 GL column | GE Healthcare | Cat# 28990944 |
| HiPrep 16/60 Sephacryl S-500 HR column | GE Healthcare | Cat# 28935606 |