Fig 5:

Antimycin A activates hTRPA1 but not hTRPV1 by ROS-dependent mechanisms in HEK293 cells. A, Mean +/− SEM [Ca²⁺]_i responses to vehicle and H₂O₂ (100μM) in non-transfected (black line), hTRPA1-expressing (green line) and hTRPV1-expressing HEK293 cells (red line). B–D, Histograms of maximal H₂O₂-evoked Ca²⁺ responses in non-transfected HEK293 (B) and HEK293 transfected with hTRPA1 (C) or hTRPV1 (D). E, Mean +/− SEM [Ca²⁺]_i responses to antimycin A (10μM) and AITC (100μM) of non-transfected HEK293 (black line), and hTRPA1-expressing HEK293 with (blue line) and without (green line) pretreatment with DTT (100μM). F, Mean +/− SEM [Ca²⁺]_i responses to antimycin A (10μM) and capsaicin (1μM) of non-transfected HEK293 (black line), and hTRPV1-expressing HEK293 with (blue line) and without (red line) pretreatment with DTT (100μM). G–I, Histograms of maximal antimycin A-evoked Ca²⁺ responses in non-transfected HEK293 (G) and HEK293 transfected with hTRPA1 (H) or hTRPV1 (I). J, Mean maximal [Ca²⁺]_i response to antimycin A in non-transfected HEK293, or HEK293 transfected with either hTRPA1 or hTRPV1 with or without DTT pretreatment. K, Mean +/− SEM [Ca²⁺]_i responses to antimycin A (10μM) and thymol (200μM) of HEK293 expressing wild-type hTRPA1 (green line) or the hTRPA1-K620A mutant (purple line). L, mean maximal [Ca²⁺]_i response to antimycin A in non-transfected HEK293, or HEK293 transfected with either hTRPA1 or hTRPA1-K620A. * denotes significant difference to non-transfected HEK293 responses (p<0.05), $ denotes significant difference to wild-type TRP responses (p<0.05), ns denotes no significant difference.