ROS scavengers inhibit antimycin A-evoked Ca2+ responses mediated by TRPA1 but not TRPV1 in vagal sensory neurons. Responses to antimycin A (10μM) in vagal neurons from TRPV1−/− mice (green columns, in A and B) and TRPA1−/− mice (red columns, in C and D) with (hatched columns) and without (clear columns) pretreatment with MnTMPyP (50 μM) and tempol (100 μM). A and C, Mean maximal [Ca2+]i responses to antimycin A of all neurons, responding nociceptors and non-responding nociceptors. B and D, percentage of all neurons that responded to antimycin A. $ denotes significant difference to responses without ROS scavengers (p<0.05), ns denotes no significant difference.