Disease incidence and activity were compared amongst control mice and mice undergoing the CIA protocol treated with weekly subcutaneous MTX at doses of 0, 2, 10, 20, or 50 mg/kg. Groups were compared based on the incidence of arthritis (A), disease activity (B), paw volume (C), and change in weight from baseline (D). Parameters were measured over the 54-day experimental period and the resulting mean±S.E.M. for each experimental group is presented.