Fig. 4.

Average nuclear volumes of cortical cells in uninoculated and inoculated roots from wild-type, dmi2–2 and dmi3–1 ROCs. Measurements were performed using the Fiji plugin 3D Object Counter. Histograms report average volumes of DAPI-stained cortical nuclei in uninoculated and inoculated ROC segments from wild-type, dmi2–2 and dmi3–1 M. truncatula ROC lines. Average nuclear volume is significantly increased in mycorrhizal compared to wild-type uninoculated cortex, as well as to uninoculated and inoculated cortical cells of dmi2–2 and dmi3–1 mutants. No statistically significant differences between samples was observed within the same lines. Bars represent standard deviations; letters indicate statstically significant differences based on Kruskal-Wallis non-parametric analysis of variance followed by Dunn’s post-hoc test (using Bonferroni-corrected p-values) for pairwise multiple comparisons (P < 0.05). Myc = inoculated; Ctr = uninoculated root cortex. The numbers in parentheses indicate the number of measurements for each experimental condition. The line inside each box represents their median, the top and bottom of each box represent upper and lower quartile respectively. Error bars represent standard deviation