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. Author manuscript; available in PMC: 2019 Nov 14.
Published in final edited form as: Chem Rev. 2018 Jul 9;118(21):10659–10709. doi: 10.1021/acs.chemrev.8b00163

Figure 11.

Figure 11

Optogenetic control of DNA recombination. (A) The programmable DNA endonuclease Cas9 was split into two parts which could be reassembled in BL-dependent manner via the Magnets LOV receptors.227 The reconstituted Cas9 enzyme mediated double-strand breaks at defined genomic sites, thus triggering non-homologous end joining and homology-directed repair. (B) Light-regulated recombination was also achieved by splitting the Cre recombinase into two fragments which were linked with AtCRY2 and AtCIB1, respectively.51,53,234 BL induced fragment assembly and restoration of activity, thereby enabling recombination at loxP sites.