Table 1.

Photophysical parameters of FbFPs

Proteina	ΦF	Brightness/ M−1 cm−1	Absmax/nm	Fluomax/nm
BsFbFP357,359	0.39	5,420	449	495
EcFbFP357,359,366	0.39; 0.44; 0.34	6,380; 4,250	448	496
Pp2FbFP357,359,366	0.17; 0.22	2,125; 3,120	449	495
Pp2FbFP-F37Sb,451	0.30	4,260c	450	497
Pp2FbFP-F37T451	0.24	3,400c	450	498
Pp2FbFP-Q116V357	0.26	3,930	439	485
Pp2FbFP-Y112L357	0.30	4,200	449	496
Pp2FbFP-L30M	0.25403	3,550c	448	494
Pp1FbFP357	0.27	3,750	450	496
DsFbFP357	0.35	5,000	449	498
iLOV128,362,366	0.32; 0.33; 0.34	4,880; 4,250	447	493
iLOV-Q489K128	0.35	5,630	440	489
miniSOG357,391	0.41; 0.37	5,820	447	497
phiLOV2.1365	0.20	2,500	450	496
MrFbFP360	0.22	3,340	448	498
TeFbFP360	0.13	1,850	445	494
YNP1FbFP360	0.31	4,120	446	496
YNP2FbFP360	0.33	4,690	449	497
YNP3FbFP360	0.20	2,840	449	498
YNP3FbFP-Y116F360	0.26	3,590	449	498
YNP4FbFP360	0.33	4,720	446	496
VfLOV361	0.23	2,875	450	498
CrLOV361	0.51	6,375	450	498
rsLOV1379	0.17	1,850	450	498
rsLOV2379	0.31	3,530	450	498
phiSOG408	0.36	4800d	449	498
phiSOG-Q103V408	0.35	4670d	444	497

^a:Organism labels: Bs = Bacillus subtilis; Ec = Escherichia coli; Pp = Pseudomonas putida; Ds = Dinoreoseobacter shibae; Mr = Meiothermus ruber; Te = Thermosynechococcus elongatus; YNP = metagenomic sequences; Vf = Vaucheria frigida; Cr = Chlamydomonas reinhardtii

^b:phenylalanine in this position is conserved in the LOV series.⁹⁰ This residue, localized on helix Cα, is in hydrophobic contact with the chromophore.^77,452

^c:calculated using the absorption coefficient of Pp2FbFP, ε₄₅₀ = 14,200 M⁻¹ cm⁻¹, reference³⁵⁷

^d:calculated using and absorption coefficient ε₄₅₀ = 13,350 M⁻¹ cm⁻¹, i.e. average between miniSOG and phiLOV2.1.^357,365,391