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Primer sequence of genes.
| Gene symbol | Primers | Amplicon (bp) |
|---|---|---|
| β-actin | F: GAAGATCAAGATCATTGCTCCT | 118 |
| R: TACTCCTGCTTGCTGATCCA | ||
| Smad2 | F: ATGGTCGTCTTCAGGTGTCTCATCGG | 257 |
| R: GGTATAGTCATCCAGAGGCGGACGTT | ||
| Smad4 | F: CGCCGTCTTCGTGCAGAGTTACTACC | 253 |
| R: GCCAGCAGCAGCAGACAGACTGATG | ||
| TGf-β1 | F:AATGGTGGACCGCAACAACGCAATCT | 102 |
| R:TCTGGCACTGCTTCCCGAATGTCTGA |
