Figure 3.

Effect of JQ1 on αSMA and β-actin protein expression and intracellular distribution. Protein expression of αSMA (a) and β-actin (b) was determined by Western blot analysis in non-ILD lung fibroblasts after 48 h in the presence or absence of TGF-β1 and JQ1(+) or JQ1(-). F-actin was separated from G-actin by differential solubility in Triton X-100. Both fractions had the same final volume, and equal volumes were run on SDS-PAGE gels. Total levels of β-actin were also analysed in whole cell extracts. Images are representative of three independent experiments. Signal intensity was measured using ImageJ. Bars represent mean ± SD from three independent non-ILD control cell lines. NS = nonsignificant. ∗p<0.05 and ∗∗ p<0.01.