Table 1.
Primers used for RT-qPCR analysis.
| Gene | Primer sequence |
|---|---|
| NOX4 | forward 5′-CTGCTGACGTTGCATGTTTC-3′ |
|
| |
| reverse 5′-CGGGAGGGTGGGTATCTAA-3′ | |
|
| |
| SOD2 | forward 5′-CACCAGCACTAGCAGCATGT-3′ |
|
| |
| reverse 5′-GAGCCCAGATACCCCAAAAC-3′ | |
|
| |
| ACTA2 | forward 5′-CCCTGAAGTACCCGATAGAACA-3′ |
|
| |
| reverse 5′-GGCAACACGAAGCTCATTG-3′ | |
|
| |
| YWHAZ | forward 5′-ACTTTTGGTACATTGTGGCTTCAA-3′ |
|
| |
| reverse 5′-CCGCCAGGACAAACCAGTAT-3′ | |
|
| |
| HPRT1 | forward 5′-TGACACTGGCAAAACAATGCA-3′ |
|
| |
| reverse 5′-GGTCCTTTTCACCAGCAAGCT-3′ | |
|
| |
| BRD3 | Applied Biosystems TaqMan probe Hs00201284_m1 |
|
| |
| BRD4 | Applied Biosystems TaqMan probe Hs04188087_m1 |
|
| |
| NRF2 (NFE2L2) | QuantiTect Primer Assay QT00027384 |
|
| |
| KEAP1 | QuantiTect Primer Assay QT00080220 |