Table 1.
Primer sequences and amplicon characteristics in the candidate reference genes.
| Gene symbol | CYORF ID | Definition | Primer sequence (5′→3′) | Amplicon | Tm | E (%) | R2 | Median Ct |
|---|---|---|---|---|---|---|---|---|
| (Forward/Reverse) | length (bp) | (°C) | ||||||
| 16S | Synpcc 7942_R0052 | 16S ribosomal RNA | F: GCAAGCCTGACGGAGCAAC | 180 | 58 | 96.3 | 0.997 | 9.13 |
| R: CGGACGCTTTACGCCCAAT | ||||||||
| petB | Synpcc 7942_2331 | cytochrome b6 | F: CTGATCCGCTCCATCCACC | 296 | 58 | 90.4 | 0.999 | 20.88 |
| R: GTTGCCTGACCGACGCTTT | ||||||||
| ppc | Synpcc 7942_2252 | phosphoenolpyruvate carboxylase | F: CCCTTGCCAGGACCAGATGA | 219 | 62 | 96.7 | 0.999 | 25.83 |
| R: CGTCGGGTGAGCGGTGAAAA | ||||||||
| ilvD | Synpcc 7942_0626 | dihydroxy-acid dehydratase | F: CGGCGCTGCGGCTCAATAT | 128 | 58 | 97.4 | 0.999 | 24.09 |
| R: ATCATCGGCGGCGACAACC | ||||||||
| prs | Synpcc 7942_2113 | ribose-phosphate pyrophosphokinase | F: TCTTGCCCTACTACGGTTACGC | 102 | 58 | 91.1 | 0.999 | 24.70 |
| R: TCGCTCCAGCCTGAGTGATTAG | ||||||||
| rnpA | Synpcc 7942_1615 | ribonuclease P protein component | F: CTCGTTGCCATCGACTGCG | 130 | 62 | 94 | 0.996 | 26.93 |
| R: CGACTTTCGTTGACGGACTCTATTT | ||||||||
| rnpB | Synpcc 7942_R0036 | RNA component of RNaseP | F: TACCGCCGATGGCCTGCTT | 119 | 58 | 105.1 | 0.998 | 16.15 |
| R: TGTCCCTCCACCTTGCTCCC | ||||||||
| secA | Synpcc 7942_0289 | preprotein translocase, SecA subunit | F: ACGACGGTCAGATTGCCGAGAT | 204 | 59.1 | 97.6 | 0.998 | 24.97 |
| R: GCGACATTCCCTGCTGGATTAG | ||||||||
| rimM | Synpcc 7942_2259 | 16S rRNA processing protein RimM | F: TGATTGACCAAGCCAGCAGCAC | 121 | 62 | 96.2 | 0.997 | 24.88 |
| R: GCCTCCACAAAGGGAATGTAGACC |