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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Dev Genes Evol. 2019 Mar 1;229(2-3):43–52. doi: 10.1007/s00427-019-00629-5

Figure 3. wnt1 CNEs are transcriptionally active.

Figure 3.

All images lateral views, 27 hpf embryos.(A,C,E,G,I,K,M,O,Q) EGFP fluorescence. (B,D,F,H,J,L,N,P,R) In situ hybridizations for EGFP transcripts. Note broad GFP fluorescence not matched by transcription pattern for CNE19 (A,B), 25 (E,F), 26 (G,H), 32 (M,N) and 33 (O,P), indicating earlier expression that terminates prior to 27 hpf. Restricted expression is observed for CNE 20 (C,D), 27 (I,J), 31 (K,L) and 37 (Q,R), but the transcription pattern for CNE 27 indicates the cessation of transcription prior to 27 hpf. In contrast, CNE 20 is transcribed robustly in the MHB and dorsal midbrain at 27 hpf. CNE 31expression in the hatching gland (K, asterisk) is likely a positional effect.