Table 2.
Method validation for all compounds in IWW including limits of detection and quantification, corrected and uncorrected matrix effects and absolute recovery and linearity. The values in brackets are the RSD (%).
| Compound | LOD (ng/L) | LOQ (ng/L) | Uncorrected Matrix Effects (%) | Uncorrected Absolute Recovery(%) | Corrected Matrix Effects(%) | Corrected Absolute Recovery (%) | Linearity (range ng/L) |
|---|---|---|---|---|---|---|---|
| 6-MAM | 0.9 | 3 | 81 (4) | 91 (4) | 99 (12) | 95 (12) | 0.9968 (0.5–17.5) |
| Alpha PVP | 0.2 | 0.6 | 62 (4) | 101 (3) | 118 (6) | 110 (2) | 0.9986 (0.5–21.5) |
| Amphetamine | 3 | 10 | 35 (7) | 111 (7) | 114 (14) | 122 (14) | 0.9976 (2–425) |
| Benzoylecgonine | 3 | 10 | 54 (3) | 137 (4) | 81 (4) | 100 (4) | 0.9984 (10–425) |
| Buprenorphine | 0.3 | 2 | 48 (1) | 99 (1) | 83 (2) | 107 (2) | 0.9983 (2–425) |
| Cocaine | 0.3 | 1 | 59 (3) | 92 (4) | 94 (2) | 112 (2) | 0.9999 (1–212.5) |
| Codeine | 20 | 50 | 76 (15) | 94 (15) | 85 (20) | 80 (20) | 0.9997 (40–8500) |
| Cotinine | 100 | 300 | 137 (17) | 117 (16) | 48 (8) | 101 (8) | 0.9996 (20–4250) |
| Ethylone | 0.02 | 0.2 | 49 (4) | 96 (4) | 81 (9) | 101 (9) | 0.9969 (0.1–21.5) |
| MDA | 0.7 | 2 | 64 (9) | 134 (8) | 82 (4) | 83 (4) | 0.9997 (2–275) |
| MDMA | 3 | 10 | 54 (6) | 96 (6) | 92 (12) | 94 (12) | 0.9997 (10–275) |
| MDPV | 0.3 | 0.8 | 57 (3) | 98 (3) | 86 (0) | 110 (0) | 0.9996 (0.5–17.5) |
| Mephedrone | 1 | 5 | 66 (23) | 85 (20) | 83 (1) | 133 (1) | 0.9993 (0.2–35) |
| Methadone | 0.3 | 1 | 42 (4) | 102 (4) | 82 (3) | 108 (3) | 0.9990 (1–212.5) |
| Methamphetamine | 15 | 50 | 52 (3) | 94 (3) | 97 (11) | 118 (11) | 0.9995 (40–1700) |
| Methcathinone | 0.8 | 2 | 44 (12) | 121 (11) | 95 (1) | 118 (1) | 0.9925 (1–35) |
| Methylone | 0.3 | 1 | 44 (7) | 90 (7) | 90 (8) | 109 (9) | 0.9989 (0.1–21.25) |
| Morphine | 30 | 100 | 94 (8) | 104 (7) | 55 (4) | 101 (4) | 0.9990 (20–4250) |
| Norbuprenorphine | 5 | 10 | 47 (3) | 101 (3) | 86 (2) | 107 (2) | 0.9996 (2–350) |
| Norfentanyl | 0.3 | 1 | 61 (8) | 93 (7) | 80 (6) | 117 (6) | 0.9995 (0.2–42.5) |
| Noroxycodone | 0.4 | 1 | 64 (10) | 102 (9) | 82 (7) | 103 (7) | 0.9990 (1–212.5) |
| Pholedrine | 3 | 10 | 47 (2) | 111 (4) | 106 (4) | 91 (4) | 0.9991 (8–1400) |
| PMA | 0.7 | 1 | 46 (29) | 90 (28) | 110 (14) | 119 (13) | 0.9964 (1–21.25) |
| TFMPP | 0.03 | 0.5 | 50 (4) | 104 (4) | 77 (15) | 101 (14) | 0.9993 (0.5–21.25) |
LOD = limit of detection; LOQ = limit of quantification.