Skip to main content
. 2019 May 1;12(6):859–870. doi: 10.1016/j.tranon.2019.03.005

Figure 2.

Figure 2

Biologic effects of metadherin overexpression in MPM.

(A) MTDH overexpression promoted proliferation in MPM cells. MPM cell lines (H2452, MSTO-211H, and H2373) were stably overexpressed with MTDH (red). Data are expressed as relative fold change compared with day 0. On day 3, cell proliferation increased 1.3-fold (H2452), 1.7-fold (MSTO-211H), and 3.0-fold (H2373) (P < .05). (B) Boyden chamber assay showed that MTDH overexpression increases invasive capability of MPM cells by 1.9-fold (H2452) and 2.6-fold (H2373). (C) Soft agar assay in which MTDH overexpression accelerated colony formation. Relative colony numbers increased 2.2-fold (H2452), 1.9-fold (MSTO-211H), and 1.6-fold (H2373) (P < .05). (D) Cell viability was assessed at varying concentrations of cisplatin (CDDP) to calculate IC50 values. MTDH overexpression (red) rendered MPM cells more chemoresistant, requiring 2.6-fold (H2452), 4.0-fold (MSTO-211H), and 3.1-fold (H2373) higher doses of drug to reach IC50 levels. (E) MTDH overexpression significantly decreased apoptosis after 72 hours of treating MTDH-induced cells with a fixed concentration of cisplatin (IC50) by 15.9% (H2452), 16.9% (MSTO-211H), and 14.0% (H2373) compared to parental cells. (F) Western blot showing decreased apoptotic proteins (cleaved PARP and caspase-3) at 72 hours of cisplatin exposure in both H2452 and H2373 MPM cells overexpressing MTDH. Where applicable, data are presented as mean ± SE. Ctrl is overexpression vector control sample. * is P < .05 versus parent cell line and/or negative control specimen. MTDH-OE is stably overexpressed MTDH in MPM cells.