Supplementary Figure S2.

Confirmation of MTDH altered expression in transfected MPM lines.

(A) Validation of MTDH overexpression constructs. The MTDH gene was stably overexpressed in three representative MPM lines H2452, MSTO-211H, and H2373 by lentiviral transfection. In all MPM lines, the MTDH protein amount markedly increased above basal levels of parental cells when the MTDH transcript was overexpressed (MTDH-OE). (B) Validation of MTDH knockdown constructs. Although CRISPR/Cas9 gene editing was used, because single clones were not selected, there remained low detection of MTDH transcripts in MPM cell lines. In all MPM lines, the MTDH protein amount markedly decreased below basal levels of parental cells when the MTDH transcript was suppressed (MTDH-KD). MTDH mRNA transcript was assessed by qRT-PCR, and protein amount was assessed by Western blotting. Where applicable, data are presented as mean ± SE. Ctrl is a negative control. * P < .05.