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. 2019 May 1;12(6):859–870. doi: 10.1016/j.tranon.2019.03.005

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Supplementary Figure S3 — Modulating metadherin expression levels affects pemetrexed sensitivity in MPM cell lines.

(A) Cell viability was assessed at varying concentrations of pemetrexed to calculate IC₅₀ values. MTDH overexpression (red) rendered MPM cells less sensitive to treatment, requiring 2.2-fold (H2452), 1.3-fold (MSTO-211H), and 2.2-fold (H2373) higher doses of drug to reach IC₅₀ levels. (B) Annexin V flow cytometry measured levels of apoptosis when H2373 cells were treated with a fixed dose (IC₅₀) of pemetrexed. Cells overexpressing MTDH (MTDH-OE) had lower levels of apoptosis. (C) Cell viability was assessed at varying concentrations of pemetrexed to calculate IC₅₀ values. MTDH knockdown (red) rendered MPM cells more sensitive to treatment with pemetrexed, requiring 5.8-fold (H2452), 2-fold (MSTO-211H), and 1.9-fold (H2373) lower doses of drug to reach IC₅₀ levels. (D) Annexin V flow cytometry measured levels of apoptosis when H2373 cells were treated with a fixed dose (IC₅₀) of pemetrexed. Cells with reduced expression of MTDH (MTDH-KD) had lower levels of apoptosis. Where applicable, data are presented as mean ± SE. Ctrl is a negative control. OE indicates gene overexpression. KD indicates gene suppression. * P < .05.