Supplementary Figure S5.

Biologic effects of metadherin suppression in MPM cells.

(A) MTDH knockdown (red) decreased proliferation in MPM cells. On day 3, cell proliferation decreased 47% (H2452), 17% (MSTO-211H), and 20% (H2373) (P < .05). (B) Boyden chamber assay showed that MTDH knockdown decreases invasive capability of MPM cells by 48% (H2452) and 63% (H2373). (C) MTDH knockdown abrogated colony formation. Soft agar assays reveal that relative colony numbers decreased 82% (H2452), 76% (MSTO-211H), and 53% (H2373) (P < .05). (D) Cell viability was assessed at varying concentrations of cisplatin to calculate IC₅₀ values. MTDH knockdown (red) rendered MPM cells more chemosensitive, requiring 24% (H2452), 52% (MSTO-211H), and 70% (H2373) lower doses of drug to reach IC₅₀ levels. (E) Levels of apoptosis were measured after MPM cells were treated with a fixed dose (IC₅₀) of cisplatin. MTDH knockdown significantly augmented apoptosis at 48 hours by 1.5-fold (H2452), 1.2-fold (MSTO-211H), and 1.3-fold (H2373) compared to parental cells. Where applicable, data are presented as mean ± SE. Ctrl is a knockdown scrambled sample. * is P < .05 versus parent cell line and/or negative control specimen. MTDH-KD is stable gene suppression of MTDH in MPM cells.