Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 18;100(2):479–494. doi: 10.1093/biolre/ioy186

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction.

This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)

PMC Copyright notice

Figure 5. — Rosiglitazone prevents the cytosolic release of cytochrome C and decreases its interaction with APAF1 under H/R condition. (A) The expression of cytochrome C in the cytosol and mitochondrion fractions isolated from JEG-3 cells exposed to H/R and rosiglitazone in comparison to normoxia (20% O₂) condition. COX IV, an inner membrane protein is used for control of mitochondrial protein loading and GAPDH used for total cell lysate loading. (B) Proximity ligation assay (PLA) showing the interaction between cytochrome C and APAF1 (red dots). Negative control (negative) imaged cells processed after treatment with rosiglitazone (Rosi.) without primary antibodies. N = 3; scale bar = 100 μm.