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. 2019 Mar 21;10(5):1051–1059. doi: 10.1111/1759-7714.13038

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© 2019 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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NFAT5 contributes to miR‐194 mediated proliferation, migration, and invasion of non‐small cell lung cancer (NSCLC) cells. (a) NFAT5 messenger RNA and (b) NFAT5 protein expression were examined in A549 and H1299 cells under high glucose (HG) or low glucose (LG) treatment by real‐time PCR and Western blot, respectively. LG, HG. (c) Quantitative real‐time‐PCR analysis of NFAT5 expression in 50 cases of NSCLC tissues with or without diabetes mellitus (DM) (**P < 0.05, Wilcoxon signed‐rank test). (d–g). Wound healing, Transwell, CCK8, or cell counting assays were used to detect the proliferation, migration, or invasion ability of A549 cells with different treatments, respectively. Additionally, the protein levels of NFAT5 were examined by Western blot. Data are reported as mean ± standard deviation of three independent experiments (*P < 0.05, t‐test).

Inline graphic — NFAT5 contributes to miR‐194 mediated proliferation, migration, and invasion of non‐small cell lung cancer (NSCLC) cells. (a) NFAT5 messenger RNA and (b) NFAT5 protein expression were examined in A549 and H1299 cells under high glucose (HG) or low glucose (LG) treatment by real‐time PCR and Western blot, respectively. LG, HG. (c) Quantitative real‐time‐PCR analysis of NFAT5 expression in 50 cases of NSCLC tissues with or without diabetes mellitus (DM) (**P < 0.05, Wilcoxon signed‐rank test). (d–g). Wound healing, Transwell, CCK8, or cell counting assays were used to detect the proliferation, migration, or invasion ability of A549 cells with different treatments, respectively. Additionally, the protein levels of NFAT5 were examined by Western blot. Data are reported as mean ± standard deviation of three independent experiments (*P < 0.05, t‐test).