Figure 6. E3 ligase RNF146 interacted with BRD7 in a PARP1‐dependent manner.

• A, B
  HeLa cells transfected with the indicated plasmids; after 24 h, cells were lysed with RIPA buffer followed by immunoprecipitation (IP) using either anti‐Myc or anti‐Flag agarose and Western blot with indicated antibody (n = 3).
• C
  HeLa and MDA‐MB‐231 cells were lysed with RIPA buffer, and lysates were subjected to IP using either anti‐IgG, anti‐BRD7 or anti‐RNF146 antibodies followed by analysis with Western blot (n = 3).
• D
  Association of endogenous BRD7 with RNF146 in HeLa cells was performed by co‐IP using anti‐RNF146 antibody. Cell lysates were incubated with protein G agarose beads conjugated with indicated antibodies, and Western blot was performed (n = 3).
• E
  HeLa cells with wild type or PARP1 knockout were transfected with Myc‐BRD7‐WT for 24 h, and their lysates were subjected to IP using anti‐Myc agarose and analysed by Western blot with indicated antibodies (n = 3).
• F
  HeLa cells stably expressing Flag‐RNF146 were transfected with either Myc‐BRD7‐WT or Myc‐BRD7‐mutant for 24 h, and lysates were subjected to IP using anti‐Flag agarose and analysed by Western blot with indicated antibodies (n = 3).

Source data are available online for this figure.