Figure 3.

Overexpression of TPT1‐AS1 remarkably enhanced the invasive and metastatic abilities of epithelial ovarian cancer (EOC) cells in vitro and in vivo. A, Adhesion assays in EOC cells with dysregulated TPT1‐AS1 expression in vitro. Scale bar, 100 μm. B, Transwell migration and invasion assays in EOC cells with dysregulated TPT1‐AS1 expression in vitro. Scale bar, 200 μm. C, The growth of the TPT1‐AS1‐overexpressing SKOV3 orthotopic ovarian xenografts was detected with a NightOWL LB 983 In Vivo Imaging System. D, After sacrifice, the ovarian tumors in the nude mice were removed, and these tumors are indicated by the red arrows in the images. E, The average number of peritoneal tumor nodules and the average weight of the tumors from each group were quantified. Data represent mean ± SD from 3 independent experiments. *P < 0.05 and ***P < 0.001