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. 2019 Mar 20;20(5):e47121. doi: 10.15252/embr.201847121

Figure EV4. Immunization with MOG, RA and IL‐2 induce T cells that express markers of Tr1 cells.

Figure EV4

  1. Representative FACS plots of CD44 expression in CD4 T cells and representative histogram of LAG‐3 expression by total CD4 and CD44+ CD4 T cells in naïve and amplified CD4 T cells, respectively. Amplified cells are LN cells of mice immunized twice with MOG, RA and IL‐2 and amplified in vitro with MOG, RA and IL‐2 for 8 days.
  2. Representative histograms of PD‐1, CD226, ICOS and CD25 expression on naïve CD4 T cells and amplified CD44+ CD4 T cells from mice immunized with MOG, RA and IL‐2.
  3. Mean fluorescence intensity (MFI) of CD62L, CD49b, ICOS and PD‐1 expression on naïve CD4 T cells and amplified CD44+ CD4 T cells from LNs of mice immunized twice with MOG (blue bars) or MOG, RA and IL‐2 (red bars) and amplified in vitro with MOG or MOG, RA and IL‐2 for 8 days. Results are mean + SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, by unpaired two‐tailed t‐test.
Data information: Cells were gated as single live CD3+ CD4+ (A, left panel), then CD44+ (A, right panel and C) and CD44+ LAG‐3+ (B). FMO: Fluorescence Minus One.