Table 1.

Analysis by Median Effect Equation of protection by sulforaphane of human retinal pigment epithelial cells (ARPE-19), keratinocytes (HaCaT), and murine leukemia (L 1210) cells, against toxicities of menadione, tert-butyl hydroperoxide, 4-hydroxynonenal, and peroxynitrite

Oxidants	Sulforaphane, μM	Dm, μM	m	r2
	ARPE-19 cells
Menadione	0.00	72.2	3.35	0.952
	0.63	98.6	3.69	0.979
	1.25	110	3.49	0.983
	2.50	123	3.58	0.994
	5.00	134	3.12	0.972
tert-Butyl hydroperoxide	0.00	95.8	2.52	0.923
	0.63	140	2.17	0.964
	1.25	163	1.79	0.980
	2.50	165	1.26	0.953
4-Hydroxynonenal	0.00	8.70	2.85	0.885
	0.63	14.1	2.51	0.931
	1.25	25.8	2.78	0.981
	2.50	26.8	2.51	0.993
Peroxynitrite	0.00	1440	6.07	0.958
	0.63	2780	6.30	0.984
	1.25	2820	6.19	0.982
	2.50	2890	6.62	0.977
	HaCaT cell
tert-Butyl hydroperoxide	0.00	63.5	0.899	0.955
	0.63	113	0.894	0.965
	1.25	166	0.921	0.971
	2.50	200	0.768	0.974
	L1210 cell
Menadione	0.00	12.2	0.725	0.967
	0.16	19.6	0.864	0.986
	0.31	26.5	1.00	0.987
	0.63	36.2	1.17	0.977

The cells were treated with the oxidants and their viability determined by the MTT reduction measurements under conditions described in the text. D_m values were obtained from a series of plots of log (f_a/f_u) with respect to log oxidant concentration at each concentration of sulforaphane. The m values are the slopes of these plots, and r² is the linear correlation coefficient.