Figure 2.

Promotion of cell proliferation, cell cycle transition, cell invasiveness, and inhibition of cell apoptosis by testis‐specific protein, Y‐linked 1 (TSPY1) in A549 lung adenocarcinoma cells and HepG2 liver hepatocellular carcinoma cells. A, Western blot analysis showed that TSPY1 was exogenously overexpressed in A549 and HepG2 cells. B, CCK‐8 assays showed that TSPY1 overexpression enhanced the proliferation of A549 and HepG2 cells. The relative proliferation is presented as the fold change, calculated based on absorbance and normalized to a control value. C, Colony formation assays showed that TSPY1 overexpression increased the colony numbers in A549 and HepG2 cells. Original magnification, ×1. D, Transwell assays showed that TSPY1 overexpression accelerated invasion of A549 and HepG2 cells. Original magnification, ×400. E, Cell cycle assays showed that TSPY1 overexpression promotes G₂/M phase transition in the cell cycle, and a shorter phase transition was found in A549 and HepG2 cells. F, Cell apoptosis assays showed that TSPY1 overexpression inhibits apoptosis of A549 and HepG2 cells. Data are presented as the mean ± SD (n = 3, *P < .05)