Figure 8.

Knockdown of testis‐specific protein, Y‐linked 1 (TSPY1) inhibits cell proliferation, cell cycle transition, and cell invasiveness and promotes cell apoptosis in LCLC‐103H lung carcinoma and MHCC97H liver carcinoma cells. A, Western blot analysis shows decreased endogenous TSPY1 in LCLC‐103H and MHCC97H cells after transfection with the lentiviral TSPY1 shRNA vector. B, CCK‐8 assays show that the knockdown of TSPY1 inhibited the proliferation of LCLC‐103H and MHCC97H cells. Relative proliferation is presented as the fold change, calculated based on absorbance and normalized to a control value. C, Colony formation assays show that the knockdown of TSPY1 decreased the colony numbers of LCLC‐103H and MHCC97H cells. Original magnification, ×1. D, Transwell assays show that the knockdown of TSPY1 could impair invasion of LCLC‐103H and MHCC97H cells. Original magnification, ×400. E, Cell cycle assays show that the knockdown of TSPY1 could extend G₂/M phase. F, Cell apoptosis assays show that the knockdown of TSPY1 could promote apoptosis of LCLC‐103H and MHCC97H cells. Data are presented as the mean ± SD (n = 3, *P < .05). shNC, Scrambled shRNA vector acting as a negative control