Figure 6.

TaBZR2 directly regulates the expression of TaGST1. A, The diagram shows the structure of the TaGST1 promoter. The sequences represent TaGST1 probe sequences. The underlined sequences indicated the core elements or mutated core elements in the TaGST1 probe. B, EMSA of TaBZR2 binding to the promoter of TaGST1. Biotin-labeled TaGST1 probes (normal and mutated) were incubated with GST or GST-TaBZR2 protein. 100× competitor fragments were added to analyze the specificity of binding. C, TaBZR2 increases TaGST1 promoter activity in wheat protoplasts. TaBZR2 was cotransfected with either TaGST1 promoter or mutated TaGST1 promoter. The LUC to REN ratio is shown and indicates the activity of the transcription factors on the expression level of the promoters. Each data point is the mean (±se) of 10 biological replicates (**P < 0.01; Student’s t test).