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. 2019 Mar 8;180(1):621–633. doi: 10.1104/pp.18.01552

Figure 5.

Figure 5.

TOX of TFs in persimmon fruit discs. The TOX experiments were conducted with two negative controls (empty vector pGreen II 002962-SK [SK] and DkERF1) and target TFs (DkERF24, DkWRKY1, and DkERF24+DkWRKY1) using persimmon fruit discs. Tissues from each of the infiltrated discs were taken to measure the soluble tannin content (A) and relative gene expression levels of related downstream genes compared with the SK (B–E) at daily intervals during the 3-d incubation. Soluble tannin content was measured with Folin-Ciocalteu reagent and quantitated by reference to standard tannin acid. Error bars indicate SEs from three biological replicates. The soluble tannin content and gene expression were compared between geneTOX and the control (empty vector) at each sampling point; **P < 0.01, ***P < 0.001.