Figure 7.

Regulatory roles of TFs involved in the regulation of the DkPDC2 promoter as part of the hypoxia response/deastringency process in persimmon fruit. DkERF9 and DkERF19 are two direct activators of the DkPDC2 promoter (Min et al., 2012, 2014). In addition, two MYB TFs, DkMYB6 and DkMYB10, were characterized as the upstream activators via binding to DkERF promoters (Zhu et al., 2018). In this study, an ERF/WRKY TF complex responsive to hypoxia and which transactivated the PDC2 promoter was found with both persimmon fruit (DkERF24 and DkWRKY1) and Arabidopsis (AtERF1 and AtWRKY53) homologs. Meanwhile, three individual DkWRKY/DkERF TFs (DkERF23, DkERF25, and DkWRKY7) also directly recognized and activated the PDC2 promoter. Arrows indicate direct interactions. The red stars indicate the transactivation effects (one star, 2- to 5-fold; two stars, >10-fold).