Figure 6.

In vitro characterization of PrCYP79D73. A, GC/Q-TOF analysis of reaction products obtained by incubation of l-Phe and NADPH with yeast microsomes prepared from PrCYP79D73 (PrCYP79D73)- or empty vector (EV)-expressing WAT11 cells. PrCYP79D73+4PI, PrCYP79D73-enriched microsomes were pretreated with 4-phenylimidazole (4PI) prior to in vitro assay. The products were confirmed by comparison of retention times and mass spectra with those of standards. TIC, Total ion chromatogram. B, TIC of authentic (E/Z)-PAOx standard. C, Head-to-tail comparison of mass spectra of the peaks from A with the authentic standard in B. m/z, Mass-to-charge ratio.