Figure 6.

syp121 complementation with SYP121^R20AR21A alone blocks secretory traffic independent of SEC11^Δ149. Images at uniform magnification are (left to right) 3D projections of fluorescence signals with the secretory marker secreted yellow fluorescent protein (secYFP), the retained (endoplasmic reticulum) marker GFP-HDEL, and bright field. The Arabidopsis syp121 mutant was complemented with SYP121, SYP121n1, SYP121n2, SYP121n3, and SYP121^R20AR21A driven by the UBQ10 promoter (Grefen et al., 2010b). The wild type and the syp121 line were included as controls. The secYFP and the endoplasmic reticulum marker GFP-HDEL were expressed with or without SEC11^Δ149 fragment by using the pTecG-2in1-CC vector (Karnik et al., 2013b, 2015). Immunoblot analysis verifying the expression of SEC11^Δ149 with the anti-myc antibody is shown at right. Bar = 100 μm.