Figure 4.

Functional complementation of SlHAK10 for K⁺ acquisition in the K⁺ uptake-defective yeast strain R5421. A, Growth status of R5421 cells expressing AtAKT1, empty vector (pYES2), and SlHAK10 on AP medium supplemented with 10, 5, 1, and 0.5 mm KCl. Each transformant was precultured in liquid AP medium containing 100 mm KCl. The 1:10 serial dilutions of yeast cells were spotted on the AP medium and then incubated at 30°C for 6 d. B, Growth kinetic analysis of the yeast R5421 cells expressing AtAKT1, empty vector, and SlHAK10 cultured in liquid AP medium containing 1 mm KCl. Errors bars represent sd of five biological replicates.