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. 2001 Dec 4;98(26):15227–15232. doi: 10.1073/pnas.261359098

Figure 4.

Figure 4

(A) Construction of recombinant gp120 fragments. The relative position of the four β strands, β2, β3, β20, and β21, is indicated in the schematic presentation of gp120. A glycine residue was added to MBri between β21 and β2 as a linker to join two gp120 fragments that span from amino acids 421–437 and 118–207 as indicated (437G118). The amino acid numbering system used here is the same as that described by Kwong et al. (22). M2–MBri is identical to MBri, except for an amino acid change that corresponds to the T198P mutation (* under the β3 region indicates this mutation). DP283 is a DH012 V3 peptide. (B). Reactivity of IgGb12 to recombinant gp120 fragments. The binding of IgGb12 to the recombinant proteins was determined by using an ELISA assay as described (25). IgGb12 (10 μg/ml) was added to ELISA plates coated with the recombinant proteins MBri or M2–MBri in the presence of various concentrations of the free MBri or M2–MBri, respectively, as indicated.