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. 2019 Feb 19;180(1):543–558. doi: 10.1104/pp.18.01503

Figure 6.

Figure 6.

D287 is critical for the plasma membrane localization of BAK1. A, Subcellular localization of BAK1-GFP (top) and BAK1D287A-GFP (bottom) in root epidermis of 5-d-old Arabidopsis seedlings. The plasma membrane was stained with FM4-64 (2 μM). B, Pearson’s correlation coefficient (r value) for the colocalization between GFP and FM4-64 fluorescence in the plasma membrane. These values were measured in 15 cells. Error bars = mean ≥ sd. P value (t test), *P < 0.01. C, Colocalization of BAK1D287A-GFP with ER marker, mCherry-HDEL. Scale bars = 20 μm. D, Leaves of 4-week-old N. benthamiana were hand-inoculated with Agrobacteria carrying 35S::BAK1-HA or 35S::BAK1D287A-HA together with 35S::PSKR1-HA. BAK1 from total proteins and enriched plasma membrane proteins were analyzed by western blot (WB) with an α-HA antibody. Cytosolic MPK6 was detected by α-MPK6 WB. The above experiments were repeated three times with similar results.