Figure 1.

ABI5 is involved in ABA-mediated Cd accumulation. A and B, Four-week-old hydroponics-grown Col-0, ABI5OX, Ws, and abi5-1 seedlings were transferred to fresh medium containing 10 µm CdCl₂ with or without 1 µm ABA for 2 d. The leaves (A) and roots (B) were harvested and subjected to Cd content analysis by inductively coupled plasma atomic emission spectroscopy (ICP-AES). Error bars represent the sd (n = 6). DW, Dry weight. C, Five-day-old Arabidopsis Col-0, ABI5OX, Ws, and abi5-1 seedlings germinated on one-half-strength Murashige and Skoog (1/2 MS) medium were transferred to fresh medium supplemented with or without 25 to 35 μm CdCl₂ for continued growth for 4 d, and the relative PR growth was measured. Three independent experiments were performed using at least 20 plants per experiment. Error bars represent the sd (n = 3). Different letters indicate that values were significantly different at P < 0.01 according to Tukey’s test.