Figure 7.

HIPP44 regulates Cd accumulation in Arabidopsis. A, HIPP44 expression patterns detected in the 2-week-old HIPP44promoter:GUS transgenic line. Bar = 1 mm. B, GUS staining of the root tips, cotyledons, and shoots of HIPP44promoter:GUS transgenic seedlings without or with 30 μm Cd for 12 h. Bars = 0.1 mm (roots) and 0.5 mm (shoots). Three independent experiments were performed using at least 20 plants per experiment with similar results. C, Four-week-old hydroponics-grown Col-0, HIPP44OX, SRDX49, and SRDX49/HIPP44OX seedlings were transferred to fresh medium containing 10 µm CdCl₂ for 2 d, and Cd contents were then determined by ICP-AES. Error bars represent the sd (n = 6). Different letters indicate that values were significantly different at P < 0.01 according to Tukey’s test. DW, Dry weight.