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. 2019 Feb 20;180(1):153–170. doi: 10.1104/pp.18.01549

Figure 8.

Figure 8.

Glutathione turnover is affected in ts-pgdh1 lines. A, Time course analysis of glutathione content in AP and roots of two ts-pgdh1 lines (ts-pgdh1.1 and ts-pgdh1.2) grown in the presence of 2 mm BSO. B, Glutathione content of plants grown in medium containing 2 mm BSO and retransferred for 24 h to BSO-free or BSO-containing medium. C, In vivo staining with monochlorobimane of glutathione in roots of plants grown in BSO (2 mM) and retransferred for 24 h to BSO-free medium. Scale bar = 20 µM. D, Ratiometric fluorescence measurements of GRX1-roGFP2 redox state in a glutathione reductase 1 background mutant (gr1.1 Ubi10:GRX1-roGFP2) in which PGDH1 was silenced (ts-pgdh1.1 Ubi10:GRX1-roGFP2, ts-pgdh1.2 gr1.1 Ubi10:GRX1-roGFP2). E, Expression of GGCT2;1 in AP and roots of ts-pgdh1 lines. Data presented are means ± se; n = 5. *Significantly different to control EV (P < 0.05, Student’s t test).