Figure 4.

Western blot analysis of VEGF expression in zebrafish eyes after MG and glucose treatments. Equal amounts of zebrafish eye extracts were analyzed by western blot using anti-VEGF and anti-actin antibodies, from three independent experiments. The immunoblot bands were quantified by densitometry analysis using ImageJ software, and the ratio to β-actin was calculated by setting the value of control as 1. The resulting relative values of VEGF are shown in histograms (right panel). Values are presented as mean ± SEM. Significant difference analyzed using the one-way ANOVA and then Student's t-test: ^∗p < 0.05; ^∗∗p < 0.01.