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. 2019 Apr 15;2019:4592701. doi: 10.1155/2019/4592701

Figure 5.

Figure 5

IFN-primed AFMSCs inhibited the proliferation of H460 cells ex vivo. (a) H460 cells (1 × 104) were cocultured for 36 hours with AFMSCs or IFN-primed AFMSCs at different target-to-effector cell ratios. The effects of IFN-primed AFMSCs on LDH releases were determined. (b) AFMSCs were exposed to the indicated combinations of cytokines (20 ng/ml each) with or without Jak1 inhibitor (15 μM)/1-MT (0.5 mM) for 12 hours, then cocultured with double the number of H460 cells in the presence or absence of anti-TRAIL antibody (20 μg/ml) for 36 hours. The cell viability of each group was determined by CCK-8 counting. (c, d) The coculture experiment was prepared as described in (b) using H460 hrGFP cells instead of H460 cells. The representative photographs under a fluorescence microscope are shown (c), and the viability of H460 cells was further confirmed using Cell Imaging Multi-Mode Reader (d). Scale bars 100 μm. The results in (b, d) were shown as a percentage of the value against AFMSC control. The results were representative of three independent experiments (∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001, n = 3).