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Formation of m8A (red) and m2,8A (blue) by Cfr wt using dithionite (squares) or the flavodoxin/flavodoxin reductase (FFR) reducing system (triangles). The reactions contained 5 μM Cfr wt, 2 mM SAM, and 100 μM 155mer RNA substrate. The FFR reducing system supports better turnover of the 155mer RNA substrate than dithionite. Error bars represent the mean ± SD of three replicates.
