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. Author manuscript; available in PMC: 2019 May 6.
Published in final edited form as: Biochemistry. 2018 Jul 23;57(30):4431–4439. doi: 10.1021/acs.biochem.8b00347

Figure 4:

Figure 4:

Representative isotopic abundance of substrate adenosine (A) and m8A (B-D) from assays performed in the presence of D2O with the 155mer (B), 16mer (C) and 7mer (D) RNA substrates after two hours. In panel B, only 5% of deuterium is observed to exchange in with the 155mer. In contrast, the 16mer and 7mer allow for greater incorporation of one and two deuterons into the product. Only a maximum of two deuterons gets incorporated into the product with the 16mer and 7mer. Isotopic abundance variation for assays performed in the presence of D2O is presented in Table S2.