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. Author manuscript; available in PMC: 2019 May 6.
Published in final edited form as: Biochemistry. 2018 Jul 23;57(30):4431–4439. doi: 10.1021/acs.biochem.8b00347

Figure 6:

Figure 6:

Representative isotopic distribution of substrate adenosine (A) and product m8A (B-D) from assays performed with [methyl-d3]-SAM, apo Cfrwt→rcn, and 155mer (B), 16mer (C) and 7mer (D) after 120 minutes. In all product spectra the majority species is d2-containing m8A (m/z 284) with smaller amounts of d1 and d0-containing products. Additionally, with decreasing substrate size, greater loss of deuterium is observed. Isotopic abundance variation for assays performed with [methyl-d3]-SAM is presented in Table S4.