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. Author manuscript; available in PMC: 2019 May 6.
Published in final edited form as: Biochemistry. 2018 Jul 23;57(30):4431–4439. doi: 10.1021/acs.biochem.8b00347

Figure 7:

Figure 7:

Reactions comparing the rate of production of m8A (closed triangles) and m2,8A (closed circles) by apo Cfrwt→rcn when using unlabeled SAM (blue) or [methyl-d3]-SAM (red). The reactions contained 10 μM apo Cfrwt→rcn, 100 μM 155mer RNA substrate, and 2 mM methyl-d3-SAM or SAM. From the ratio of the slopes, an apparent kinetic isotope effect of 1.65 ± 0.17 can be extracted. Error bars represent the mean ± SD of three replicates.