Figure 6.

Effects of Cyr61 on Ang II-induced apoptosis in HEK293T cells. To investigate the effects of Cyr61 on Ang II-induced apoptosis on HEK293T cells (a). the HEK293T cells were treated with Ang II after knocking down the Cyr61 gene for 48 h, and apoptosis was measured by Annexin V-FITC/PI staining and flow cytometry. (b). The ratio of apoptotic cells to the total number of cells. The number of apoptotic cells equals the sum of the cells in the Q2 (early-stage cell apoptosis rate) and Q3 (late-stage cell apoptosis rate). ^aP < 0.05 compared with controls, ^bP < 0.05 compared with Cyr61 knockdown, ^cP < 0.05 compared with Ang II without Cyr61 knockdown, and ^dP > 0.05 compared with controls. The data represent means ± SD from 3 independent experiments.