. Author manuscript; available in PMC: 2019 Jul 20.

Published in final edited form as: Methods Enzymol. 2018 Jul 20;606:241–268. doi: 10.1016/bs.mie.2018.06.005

Table 1.

Composition of M9 Minimal Medium

1 L of 20 × M9 Salts^a	g
Na₂HPO₄ (Anhydrous)	136
KH₂PO₄	60
NaCl	10
NH₄Cl	20
Volumes for 4 L of M9 Minimal Medium
20 × M9 Salts^b	200 mL
40% w/v Glucose^c	40 mL
1 M CaCl₂^c	0.4 mL
1 M MgSO₄^c	8 mL
ddH₂O^b	3700 mL

After salts dissolve, adjust the pH of the medium to 7.4 with HCl and KOH; adjust volume to 4 L with water.

Medium should be autoclaved after addition to ddH₂O in a 6-L Erlenmeyer flask.

Solutions should be sterilized by filtration and added just before inoculation.