Table 1.
Immunohistochemical markers of lymphatics and other cells in mouse lungs
| Cell type | Marker | Host | Vendor | Catalog # | Comments |
|---|---|---|---|---|---|
| Lymphatic endothelial Cells | EGFP in Prox1-EGFP mice | Chicken | Aves | GFP 1020 | Overall best marker Strong signal. Marks cytoplasm and nucleus |
| Prox1 | Rabbit Goat | AngioBio R&D Systems | 11–002P AF2727 | Nuclei of lymphatic endothelial cells, neuroendocrine cells, and cardiac muscle cells | |
| VEGFR-3 | Goat | R&D Systems | AF743 | Good, but weaker signal and higher background than Prox1 | |
| CCL21 | Goat | R&D Systems | AF469 | In Golgi apparatus of lymphatic endothelial cells | |
| Neuropilin-2 | Goat | R&D Systems | AF564 | Endothelial cells of lymphatics and venules | |
| Podoplanin | Syrian hamster | AngioBio | 11–033 Clone 8.1.1 | Not recommended for the lungs. Expressed by lung and tracheal epithelial cells. | |
| LYVE-1 | Rabbit Rat Goat | AngioBio R&D Systems R&D Systems | 11–034 MAB2125 AF2125 | Not recommended for the lungs. Strongly expressed by blood vessels in lung [18], but not in trachea. | |
| Blood vessel endothelial cells | Pecam1 (CD31) | Armenian hamster Rat | Thermo BioLegend | MA3105 102502 | Clone 2H8 Clone MEC13.3 Weaker expression also on lymphatic EC |
| Endothelial cells | VEGFR-2 | Goat | R&D Systems | AF644 | Weaker expression also on lymphatic EC |
| High endothelial venules (HEV) | Rat (IgM) | BD Bioscience | 553863 | Found in bronchoalveolar lymphoid tissue (BALT) | |
| Smooth muscle cells | α-smooth muscle actin | Mouse | Sigma Sigma | F3777 (FITC) C6198 (Cy3) | Marks large airways and blood vessels |
| Pericytes | NG2 | Rabbit | Millipore | Ab5320 | |
| Epithelial cells (pan) | E-cadherin Pan- Cytokeratin | Rat Rabbit | Thermo Dako | 13–1900 Z0622 | Clone ECCD-2 |
| Clara/Club cells | CCSP | Rabbit | Millipore | 07–623 | |
| Ciliated cells | Acetylated tubulin | Mouse | Sigma | T7451 | Clone 6–11B-1 |
| Alveolar Type 1 cells | Aquaporin-5 | Rabbit | Abcam | Ab78486 | |
| Alveolar Type 2 cells | SPC | Rabbit | Seven Hills | WRAB-09337 | |
| Leukocytes (pan) | CD45 | Rat | BioLegend | 103102 | Clone 30-F11 |
| T-lymphocytes | CD3e | Armenian hamster | BioLegend | 100302 | Clone 145–2C11 |
| B-lymphocytes | B220 | Rat | BioLegend | 103202 | Clone RA3–6B2 |
| Neutrophils | S100A8 A100A9 | Goat Rabbit | R&D Systems Abcam | AF3059 Ab105472 | |
| Macrophages | Iba1 F4/80 | Rabbit Rat | WAKO BioLegend | 019–19741 123102 | Also known as Aif1 Clone BM8 Better in wax sections |
| Dendritic cells | CD11c | Rat | eBioscience | 14–0114-81 | Clone N418 Better in wax sections |
| Fibroblasts | S100A4 | Rabbit | Abcam | Ab27957 | |
| Mesothelial cells | Mesothelin | Goat | Santa Cruz | Sc-27702 |