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Molecular Sieve Chromatography. (A) Chromatogram of the suite of standards used to generate the standard curve as follows: (A) Blue Dextran, 2000 kDa; (B) β-Amylose, 200 kDa; (C) Alcohol Dehydrogenase, 150 kDa; (D) Bovine Serum Albumin, 66 kDa; (E) Carbonic Anhydrase, 29 kDa; (F) Cytochrome C, 12.4 kDa.
(B) Standard curve generated from the elution volume of each of the standards corrected for the void volume of the column versus the log molecular weight of each of the standards.
