. Author manuscript; available in PMC: 2019 May 6.

Published in final edited form as: Methods Enzymol. 2018 Jul 19;606:217–239. doi: 10.1016/bs.mie.2018.06.006

Table 2.

Parameters for LC-MS/MS analysis of ³⁴S-labeled reaction intermediate and product.

Compound	Parent Ion	Fragmentation Voltage	Product Ion 1	Product Ion 2
Octanoyl Peptide	932.5	240	552.3 (37)	210.1 (51)
³²S-6-thio-octanoyl Peptide	964.5	240	584.3 (37)	242.1 (51)
³⁴S-6-thio-octanoyl Peptide	966.5	240	586.3 (37)	244.1 (51)
³²S/³²S Lipoyl Peptide	996.5	240	616.4 (37)	274.1 (51)
³²S/³⁴S Lipoyl Peptide	998.5	240	618.4 (37)	276.1 (51)
³⁴S/³⁴S-Lipoyl Peptide	1000.5	240	620.4 (37)	278.1 (51)
AtsA Peptide (IS)	474.4	135	229.1 (12)	153.1 (26)
Tryptophan (IS)	188	130	146.1 (10)	118.0 (21)

Respective collision energies are in parentheses